(not for touchscreen)
As a general note I'm going to avoid making further changes unless really necessary as the map is not meant to be exhaustive but rather a general overview of the history of the Orient Express with selected services and stops.
I removed the purple and yellow lines as I was not sure wether they were correct and thought they lacked the historic character a bit and they did not reach Athens or Istanbul nor did they add any cities on the route. I coincidentally updated my quiz on the same day this one was released as I updated a few of my quizzes in light of recent spotlight update. Only thing I changed to the map that update was removing ceuta as it's floating in the sea and Milan seems to me a bit mispositioned (which still seems to be the case in this map). Although your quiz is less in jetpunk style, it does look nice and keeping the box with extra info about the lines maybe is a better idea than removing it.
The Venice-Simplon line is still running, as OfficerDibble pointed out, and I've extended it to London (it has other routes too but that's the main one). The OBB run line was the last use of what could be considered the original Orient Express according to an article I found so though it doesn't add more cities it feels right to include it for this particular quiz, which is supposed to be an overview of the history as well as a bit of a tribute to it. Have been meaning to add some information on a sticky but need to read a bit more first. Also mean to swap the purple and yellow lines in the legend to reflect the change in dates. Anyway, apologies again. I hope you feel the differences are enough.
Lovely quiz though, thanks!