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question
Answer
in dialysis what is the name of the solution that remains in the tube/bag?
retentate
what is the name of the solution that goes through the membrane in dialysis ?
filtrate
what type of ultrafiltration uses force to pull anything below the MWCO through the membrane ?
centrifugal
........ ultrafiltration uses argon gas to push the liquid through a filter at the base of the chamber
pressure
in TLC, silica gel, cellulose or aluminia are the ..........phase
stationary
what is the more precise version of chromatography that uses a chamber to keep a constant temperature and pressure ?
HPTLC
the Rf value is always less than ...
one
what does FPLC stand for
fast protein liquid chromatography
question
Answer
which type of FPLC uses dyes which mimic the substrate or cofactors of specific proteins causing the target proteins to remain on the stationary phase (column)
dye ligand
what is the name of the common blue dye used in dye ligand affinity
cibacron blue F3GA
what amino acid has a high affinity for cobalt and nickel ions and is therefore used in FPLC - metal affinity
histadine
The his tagged proteins are removed from the column when flushed with ........
imidazole
what is added to proteins in FPLC hydrophobic interaction in a small amount so that the protein is in solution but if too much is added it will precipitate ?
ammonium sulfate
in FPLC hydrophobic interaction, what 2 things are commonly added to the resin in the column
benzene rings, branched alkane side chains
what happens to the target proteins in FPLC hydrophobic interactions when the column is washed with a low concentration of salt buffer