question | Answer | % Correct |
---|---|---|
what does FPLC stand for | fast protein liquid chromatography | 100%
|
what is added to proteins in FPLC hydrophobic interaction in a small amount so that the protein is in solution but if too much is added it will precipitate ? | ammonium sulfate | 50%
|
which type of FPLC uses dyes which mimic the substrate or cofactors of specific proteins causing the target proteins to remain on the stationary phase (column) | dye ligand | 50%
|
what happens to the target proteins in FPLC hydrophobic interactions when the column is washed with a low concentration of salt buffer | fall off | 50%
|
what is the name of the solution that goes through the membrane in dialysis ? | filtrate | 50%
|
what amino acid has a high affinity for cobalt and nickel ions and is therefore used in FPLC - metal affinity | histadine | 50%
|
what is the more precise version of chromatography that uses a chamber to keep a constant temperature and pressure ? | HPTLC | 50%
|
the Rf value is always less than ... | one | 50%
|
........ ultrafiltration uses argon gas to push the liquid through a filter at the base of the chamber | pressure | 50%
|
in dialysis what is the name of the solution that remains in the tube/bag? | retentate | 50%
|
in TLC, silica gel, cellulose or aluminia are the ..........phase | stationary | 50%
|
in FPLC hydrophobic interaction, what 2 things are commonly added to the resin in the column | benzene rings, branched alkane side chains | 0%
|
what type of ultrafiltration uses force to pull anything below the MWCO through the membrane ? | centrifugal | 0%
|
what is the name of the common blue dye used in dye ligand affinity | cibacron blue F3GA | 0%
|
The his tagged proteins are removed from the column when flushed with ........ | imidazole | 0%
|
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