Bacterial biochemical test patterns

Demonstrate how each organism reacts with each test

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Last updated: August 22, 2014

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First submitted	August 20, 2014
Times taken	40
Average score	33.6%
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most test answere will be pos/neg. morphology is coccus, bacillus, branching filamentous, antibiotics are resistant or susceptible. oxygen requirements are obligate aerobe/anaerobe, facultative a/an, or microaerophillic.

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Organism	Hint	Answer
Staphylococcus aureus	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	pos
	coagulase	pos
Staphylococcus epidermidis	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	pos
	coagulase	neg
	oxidase	neg
	novobiocin	susceptible
Staphylococcus saprophyticus	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	pos
	coagulase	neg
	oxidase	neg
	novobiocin	resistant
Micrococcus spp.	gram	pos (variable)
	morphology	coccus
	oxygen requirement	obligate aerobe
	catalase	pos
	oxidase	pos
	bacitracin	susceptible
	furazolidone	resistant
Peptostreptococcus spp.	gram	pos
	morphology	coccus
	oxygen requirement	obligate anaerobe
Streptococcus pyogenes (Lancefield group A)	gram	pos
	mrophology	coccus
	oxygen requirement	microaerophile
	catalase	neg
	hemoylsis pattern	beta
	bacitracin	susceptible
	PYR	pos
Streptococcus agalactiae (group B)	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	beta
	bacitracin	resistant
	PYR	neg
	CAMP	pos
	Hippurate	pos
Streptococcus pneumoniae	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	alpha
	optochin	susceptible
viridans group Streptococcus spp.	gram	+
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	alpha
	optochin	resistant
Enterococcus faecium (group D)	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	gamma (sometimes alpha)
	bile-esculin	pos
	PYR	pos
	growth in NaCl at 37C	pos
	arabinose	pos (yellow)
Enterococcus faecalis (group D)	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	gamma (sometimes alpha)
	bile esculin	pos
	PYR	pos
	growth in NaCl at 37C	pos
	arabinose	neg (purple)
Streptococcus bovis (group D)	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	catalase	neg
	hemolysis pattern	gamma (sometimes alpha)
	bile esculin	pos
	PYR	neg
	growth in NaCl at 37C	neg
Nocardia spp.	gram	pos (weak)
	morphology	beaded, branching filamentous bacilli
	oxygen requirement	obligate aerobe
	acid fast stain	partial
	catalase	pos
Actinomyces spp. (israeilii)	gram	pos
	morphology	branching filamentous bacilli
	oxygen requirement	facultative anaerobe (grows best anaerobic)
	acid fast stain	neg
Bacillus anthracis	gram	pos (variable)
	morphology	bacillus
	oxygen requirement	facultative anaerobe
	spore formation	yes
	motility	no
	hemolysis pattern	gamma
	catalase	pos
Bacillus cereus	gram	pos
	morphology	bacillus
	oxygen requirement	facultative anaerobe
	spore formation	yes
	motility	yes
	hemolysis pattern	beta
Listeria monocytogenes	gram	pos
	morphology	bacillus
	oxygen requirement	facultative anaerobe
	spore formation	no
	hemolysis pattern	beta (narrow zone)
	motility	yes (tumbling)
	catalase	pos
	CAMP	pos
	hippurate	pos
	growth at 4-10C	pos
Erysipelothrix rhusiopathiae	gram	pos
	morphology	coccus
	oxygen requirement	facultative anaerobe
	spore formation	no
	catalase	neg
	oxidase	neg
	TSI	H2S production
	motlity	no
Corynebacterium diphtheriae	Gram	pos
	morphology	bacillus (clubbed)
	oxygen requirement	facultative anaerobe
	spore formation	no
	catalase	pos
	motility	no
	selective growth medium	tellurite/tinsdale (black colonies with a brown halo)
Clostridium spp. (diphtheriae, tetani, botulinum, perfringens)	gram	pos
	morphology	bacillus
	oxygen requirement	obligate anaerobe
	spore formation	yes

Level 82

Jul 27, 2017

Good quiz, but it completely ignores the Enterobacteraceae, a very large and very clinically significant family that includes (but is not limited to) Escherichia (E. coli), Salmonella, Shigella, Serratia, Klebsiella, Proteus, Enterobacter, Citrobacter and Yersinia (Y. pestis being the presumed cause of the dreaded, deadly plague in the middle ages). Also missing are such clinically significant genus/species such as Neisseria and Pseudomonas.

Level 32

Sep 14, 2018

Correct. It does ignore everything you mention. However, you should have noticed that this is ONLY gram positive organisms. I built it to study Gram pos organisms. In retrospect, it's not that useful in learning it.

Dr. E

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