Statistics for forensic toxicology - analytical techniques - HPLC

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Answer Stats

Hint	Answer	% Correct
	a buffer to aid separation (e.g. formic acid)	0%
	a column	0%
	a detector	0%
what is SPE?	a mini form of chromatography used to purify or concentrate the samples before they can be analysed (it also has a mobile & stationary phase)	0%
	an injector	0%
what are the 4 basic parameters of a HPLC system?	a pump	0%
what is reversed-phase HPLC?	a type of HPLC that has a non-polar stationary phase & a moderately-polar mobile phase	0%
what is normal-phase HPLC?	a type of HPLC that has a polar stationary phase & a non-polar mobile phase	0%
column	be porous to increase surface area for separation	0%
what is the most common reversed-phase HPLC?	C18 (silica with 18-carbon chains attached to the surface)	0%
what kind of data do they generate?	chromatograms	0%
column	contains the stationary phase	0%
detector	convert the data into useful information	0%
what is this type of HPLC used for?	diagnosis (clinical biochemistry)	0%
how does this separate the components?	different components of the mixture will separate based on partitioning between the stationary and mobile phases	0%
what are the most common types of detectors for HPLC?	diode array detectors (DAD), a type of UV/vis detector	0%
injector	draw the sample from a vial into a needle (load position)	0%
	either acetonitrile or methanol (non-polar-ish)	0%
column	have an internal diameter (ID) to determine sensitivity & analyte loading	0%
column	have stationary phase beads to generate pressure	0%
what is HPLC?	high performance liquid chromatography	0%
how does this separate the components?	hydrophilic molecules will be retained by the stationary phase & eluted when the mobile phase gradient changes from non-polar to polar	0%
how does this separate the components?	hydrophobic molecules will be retained by the stationary phase & eluted when the mobile phase gradient changes from polar to non-polar	0%
injector	inject the sample into the flow path of the mobile phase (inject position)	0%
injector	introduce the sample	0%
how does this help HPLC?	it provides a more pure sample for HPLC to analyse	0%
what does it entail?	it takes a pressurised liquid (the mobile phase) and passes it through a column that is filled with an absorbent material (the stationary phase)	0%
	manufacturing quality control	0%
	mass spectrometer	0%
pump	mix the solvents together effectively	0%
pump	move the mobile phase under pressure	0%
	pharmaceutical analysis	0%
what do each of these need to do?	pump the solvent	0%
what is the most common sample preparation technique used for HPLC?	solid phase extraction (SPE)	0%
pump	split the appropriate gradient (produce the right ratio of solvents used)	0%
how does this separate the components?	the components will separate based on the interaction with the sorbent material	0%
what do they involve?	the mixture being dissolved in fluid	0%
	the mixture being passed through a solid material	0%
what are the two phases in chromatographic techniques?	the mobile phase	0%
	the stationary phase	0%
what are chromatographic techniques?	those that involve the separation of mixtures	0%
how do we analyse these to make this data quantitative?	using peak area & internal standards	0%
what is the mobile phase typically made of in HPLC?	water (polar)	0%