separation techniques 2

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Last updated: January 18, 2023

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First submitted	January 18, 2023
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question

Answer

what type of FPLC used a specific buffer pH and a resin with basic side chains ?

ion exchange

when creating a salt gradient in FPLC ion exchange we mix buffer 'a' with buffer 'b' (e.g. 1MNaCl), weak bound proteins will fall off at a lower concentration than target proteins true or false ?

true

FPLC gel filtration is usedinstead of the other FPLC methods when the protein is damaged by ....

salt

in FPLC gel filtration what size proteins falls through the column first ?

large

what blue molecule is commonly used as a marker for large proteins in FPLC gel filtration ?

blue dextran

what pink molecule is commonly used as a marker for small proteins in FPPLC gel filtration?

vitamin b12

what separation technique is best suited to small biological molecules ?

HPLC

what type of HPLC consists of a non polar solvent and a polar stationary phase ?