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Statistics for separation techniques- electrophoresis

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This quiz has been taken 1 time
The average score is 11 of 15

Answer Stats

questions	Answer	% Correct
^ whereas in electrophoresis large molecules elute .....small molecules	after	100%
which hydrogel is usually used for nucleic acids or very large proteins ?	agarose	100%
negatively charged ions are known as ...... and positively charged ions are known as .......	anions cations	100%
in fplc gel filtration large molecules elute ......small molecules	before	100%
RNA must be ......... so that it does not loop up, this can be done using formaldehyde	denatured	100%
polyacrylamide gels come in two types slabs or ........(good resolution all the way down)	gradients	100%
which hydrogel is usually used for proteins (PAGE)	polyacrylamide	100%
which 2 molecules is the outdated method of paper electrophoresis NEVER used for ?	proteins, dna	100%
what is the name of the most common protein electrophoresis technique ?	SDS PAGE	100%
what is the name of the catalyst that is used in the chemical polymerisation of PAGE ? hint - it cannot work in the presence of oxygen therefore a vacuum must be used	TEMED	100%
DNA electrophoresis is done horizontally whereas protein electrophoresis is done ..........	vertically	100%
what is often overlaid to exclude oxygen in protein electrophoresis ?	butanol	0%
RNA is extremely unstable therefore must be dealt with in ultra clean conditions, in order to destroy RNase the buffer must be treated with .... and then autoclaved	DEPC	0%
what is added to the buffer in dna electrophoresis to bind to the magnesium in dna leaving it with a negative charge ?	EDTA	0%
what can be added to the loading buffer in dna electrophoresis to make the solution more dense ( keeping solution in specific well) ?	sucrose	0%

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