separation techniques- electrophoresis

Quiz by DaisyStradling
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Last updated: January 16, 2023
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First submittedJanuary 16, 2023
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questions
Answer
which 2 molecules is the outdated method of paper electrophoresis NEVER used for ?
proteins, dna
negatively charged ions are known as ...... and positively charged ions are known as .......
anions cations
which hydrogel is usually used for nucleic acids or very large proteins ?
agarose
which hydrogel is usually used for proteins (PAGE)
polyacrylamide
in fplc gel filtration large molecules elute ......small molecules
before
^ whereas in electrophoresis large molecules elute .....small molecules
after
what can be added to the loading buffer in dna electrophoresis to make the solution more dense ( keeping solution in specific well) ?
sucrose
what is added to the buffer in dna electrophoresis to bind to the magnesium in dna leaving it with a negative charge ?
EDTA
questions
Answer
RNA must be ......... so that it does not loop up, this can be done using formaldehyde
denatured
RNA is extremely unstable therefore must be dealt with in ultra clean conditions, in order to destroy RNase the buffer must be treated with .... and then autoclaved
DEPC
DNA electrophoresis is done horizontally whereas protein electrophoresis is done ..........
vertically
polyacrylamide gels come in two types slabs or ........(good resolution all the way down)
gradients
what is the name of the catalyst that is used in the chemical polymerisation of PAGE ?
hint - it cannot work in the presence of oxygen therefore a vacuum must be used
TEMED
what is often overlaid to exclude oxygen in protein electrophoresis ?
butanol
what is the name of the most common protein electrophoresis technique ?
SDS PAGE
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