object(stdClass)#211 (6) { ["pageType"]=> string(4) "quiz" ["pageId"]=> int(1010201) ["pageOwner"]=> int(31243) ["pageUrl"]=> string(0) "" ["language"]=> string(7) "english" ["disabled"]=> bool(false) } DNA technology (CCEA AS level Biology)
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DNA technology (CCEA AS level Biology)

This information is taken from AS level biology booklets so will be basic answers
Quiz by Biologist18
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Last updated: January 8, 2013
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First submittedJanuary 8, 2013
Times taken771
Average score35.0%
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Hint
Answer
The full name of DNA
Deoxyribonucleic Acids
What does the genetic code carried by DNA determine?
Which proteins the cell makes
Nucleic acids are made up of...
Nucleotides
There are two groups of bases, purines and...
Pyrimidines
Which base replaces thymine in RNA
Uracil
What kind of sugar (based off of carbon number) is in DNA and RNA?
Pentose sugar
What base does cytosine pair with?
Guanine
What is the shape of DNA
Double helix
The two strands of DNA run ____________ to each other
Antiparallel
What is the bond between the sugar and the phosphate called?
Phosphodiester
Hint
Answer
What type of RNA is made in the nucleolus?
Ribosomal RNA
How does DNA replicate?
Semi-conservative replication
What experiment proved that DNA replicated this way?
Meselson-Stahl
What percentage of new DNA is original?
50%
What reaction is taq polymerase used in?
Polymerase chain reaction
What temperature is the target DNA heated to during the above reaction to break the hydrogen bonds holding it together? (In celsius)
Annealing Temperature
A strand of single-stranded DNA of a known base sequence, labelled with either a radioactive or a fluorescent marker is called a...
DNA probe
What is it called when the above answer forms hydrogen bonds with its target DNA?
Hybridisation
What nucleotide sequence is a variation in the DNA sequence of a genome which can be used to fragment DNA by using a resriction enzyme?
Restriction fragment length polymorphisms
What is used to seperate the fragments created by fragmentation of the above?
Gel electrophoresis
Mikethemangler
+3
Level 20
Nov 11, 2013
Just a couple comments:

-Pyrimidine is misspelled, which prevented me from getting the right answer.

-The question "What kind of sugar is in DNA and RNA?" is somewhat broad. I kept trying to answer "ribose," "deoxyribose," "5-carbon sugar," I suppose I didn't think of the fact they were pentoses

-The temperature needed to break the hydrogen bonds in a DNA double helix is sequence dependent, perhaps "annealing temperature" would be a better answer to that question.

-Was somewhat frustrated that "probe" wasn't accepted for "DNA probe"

-Might want to accept "Hybridization" for those of use who speak American English.

-RFLPs are just one type of polymorphism. I kept trying to answer single nucleotide polymorphism. Perhaps I wasn't reading the question carefully enough

thorzotte
+1
Level 35
Jan 9, 2016
Agree with everything Mikethemangler says with the exception of "annealing temp". I think he means "denaturing" temperature.
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